Here's a jigsaw puzzle activity on the autonomic nervous system. Students will review the location, the neurotransmitters, the groups of nerves, the responses, and the receptors associated with the sympathetic and parasympathetic nervous systems. Students will start with a blank slate, and the jigsaw puzzle pieces will be revealed with correct answers. This activity is an auto-grading response validation Google Form with 16 questions. A print version and answer key are included.